Zebrafish models of pseudourydilation-related disorders

Description of the research topic

Pseudouridylation is one of the most common epitranscriptomic modifications in the RNA of eukaryotic cells. Despite its abundance, we still do not understand its biological relevance completely. Nevertheless, impairment of this process can lead to severe, recessive disorders. During this doctoral project the candidate will participate in the creation and analysis of multiple zebrafish (Danio rerio) disease models related to the defect of pseudouridylation. Bowen-Conradi Syndrome (BCS) results from a missense mutation of the Essential for Mitotic Growth 1 (EMG1) gene, whereas MLASA (Mitochondrial myopathy and sideroblastic anemia) is caused by the impairment of the Pseudouridine Synthase 1 (PUS1) gene. During the project we will use genome editing technique to create the relevant mutations in these genes, and analyze the resulting zebrafish strains using molecular and histochemical methods.

Thesis supervisor: Máté Varga

Required language skills: English and Hungarian
Further requirements: Earlier experience with zebrafish.

How to Apply?

If you are interested apply here: [PhD] Doctoral School of Biology – Eötvös Loránd University (elte.hu)

For more information visite the following website: Doctoral School of Biology (elte.hu)

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